He broke the previous world record set in Berlin by Kenyan Dennis Kimetto in 2014 by 1 minute and 18 seconds an improvement that had not been witnessed in fifty years.
There were also early signs that a record break was on the cards as Kipchoge ran the first kilometer in a meagre 2:43.
Kenya's Gladys Cherono also smashed the Berlin marathon coming first in the women's race after running 2:18:10 (unofficial) and setting a new course record and world-leading time.
"They say you can miss it twice but not third time.
So I want to thank everyone who has helped me", said Kipchoge, who a year ago took part in the Nike Breaking Two project, where he ran 2:00:25 with the aid of "illegal" in and out pacemakers.
"To be precise, I am just going to try to run my personal best".
Kipchoge came agonisingly close to sporting immortality by almost running the first sub two-hour marathon previous year.
Eliud Kipchoge smashed the world marathon record by more than a minute
Kipchoge, who past year took part in the Nike Breaking Two project, where he ran two hours and 25 seconds with the aid of "illegal" in and out pacemakers, started off at a sizzling pace.
His three pacemakers were pushed to the limit to keep the tempo high as Kipchoge dipped well below world-record time by the halfway mark.
"Kipchoge hit the half way mark with one pacesetter in 57:26 minutes, way inside Kimetto world record by one minute and nine seconds before hitting 1:26:45 against Kimetto's 1" 27:38 at 30km mark.
The German capital has now seen the last six men's world records over the distance set on its quick course.
"It was hard running alone, but I was confident". I had to focus on the work I had put in in Kenya and that is what helped push me.
This latest triumph is certain to earn Kipchoge - considered the greatest marathon runner of all time - atleast Sh100 million in prize money and bonuses.
Fellow Kenyan Amos Kipruto came second in 2 hours, 6 minutes and 23 seconds, followed by a third Kenyan, former world-record holder Wilson Kipsang, who was 25 seconds behind.
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